A novel antibiotic drug delivery mechanism
Summary :
Table of Contents
- Abstract
- Introduction
- Discussion on domain insertion technique in creating molecular switches
- Targeted antibiotic drug delivery
- Conclusion
- References
Abstract
On the most basic level, protein engineering entails changing an existing protein sequence for a new purpose.1 Although a rather young field, the last decade has seen a giant leap in the understanding of protein structure and function. The advent of DNA shuffling in 1994, for example, has led to increased activity, thermodynamic stability, and folding in certain enzymes.2 Despite great advances in improving the activities of certain proteins, engineering proteins with new biomolecular functions has largely remained beyond scientists' capabilities.Two approaches towards protein engineering have been applied, consisting of both rational and evolutionary engineering designs.3 The former generally necessitates an extensive knowledge of the protein's structure, active site, mechanism and co-factors in order to reach a desired goal. These techniques have predictably had much difficulty in creating proteins with new functions. Evolutionary approaches, however, seem to be the future of protein engineering. These methods consist of using nature's principle of creating diversity through mutations and then designing selection methods to find proteins with a desired function.
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