Bacterial Transformation Lab Report

Type :

Case study

Pages :

2 pages

Format :

.doc

Published date :

10/23/2007

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Summary :

 
 

Table of Contents Bacterial Transformation Lab Report Table of Contents

 
  1. Introduction
  2. Methods
  3. Results
  4. Discussion
  5. Conclusion
  6. Works cited

Abstract

bacterial transformation is the transforming of a bacterial cell using plasmids spliced with various types of DNA from other cells (Miyazaki, 201). Instead of a nucleus with chromosomes, bacterial cells have one large circular chromosome in their cell. They also have much smaller rings of DNA throughout their cytosol. These smaller DNA rings are the plasmids which one can manipulate and introduce into cells for transformation to occur. (Miyazaki, 203)
One example of such a manipulated plasmid is the pGLO plasmid. This is the plasmid that we used in this lab. We introduced this plasmid into E. Coli bacteria using the heat shock method. This method involves placing the transformation mixture with the bacteria and the plasmid into ice and then into a hot water bath several times. We also made use of a CaCl2 solution which made the cell walls of the cells even more permeable to the plasmids.

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About the author :

pencil image Michael G.  
Level :General public Study : Medical studies School/University : SUNY at Stony Brook

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