Bioreactor: Optimizing E.Coli fermentation for Bacteria directed enzyme prodrug therapy
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Abstract
E.coli strain WM04 was determined as a viable option for bacteria directed enzyme prodrug therapy. Determination of suitability for animal testing depends on sufficient quantities of bacteria being produced through fermentation. Optimal operating conditions are found for the production of WM04 by observing two important production variables: the quantity of bacteria produced and the capacity for survival of the bacteria.
One limitation to bacterial growth is the amount of oxygen supply. The concentration of dissolved oxygen and oxygen transfer rates are correlated to cell growth. Impellor velocity, ranging from 200-1000 rpm, allowed us to determine the optimal speed at which the greatest oxygen transfer rate was achieved in the bioreactor solution with minimal bacterial shearing.
The dissolved oxygen content of the media in the bioreactor was measured with an oxygen probe at varying impellor speeds. BioCommand Lite software was used to plot the dissolved oxygen concentration against the time of the run and the impellor speed versus time.
One limitation to bacterial growth is the amount of oxygen supply. The concentration of dissolved oxygen and oxygen transfer rates are correlated to cell growth. Impellor velocity, ranging from 200-1000 rpm, allowed us to determine the optimal speed at which the greatest oxygen transfer rate was achieved in the bioreactor solution with minimal bacterial shearing.
The dissolved oxygen content of the media in the bioreactor was measured with an oxygen probe at varying impellor speeds. BioCommand Lite software was used to plot the dissolved oxygen concentration against the time of the run and the impellor speed versus time.
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