Determination of various protein concentrations by means of spectrophotometry
Summary :
Table of Contents
- Introduction
- Methods
- Results
- Sample calculations
- Standardized concentration calculations
- Lowry Assay concentration calculations
- UV Direct Assay concentration calculations
- Discussion
- Conclusion
- References
Abstract
spectrophotometry relies on the varying capabilities of molecules to absorb photons of light. This absorbance is characteristic of specific compounds, and can therefore be utilized to quantify and classify the presence/quantities of various compounds. (Harris, 2007) With the use of standard calibration curves, the concentrations of various samples can be graphically interpreted as a function of the absorbance. The relation between absorbance and concentration is governed by the Beer-Lambert law, which states that the absorbance changes proportionally with the concentration. (Harris, 2007) This fundamental principle allows for a direct correlation between the examined absorbances and the corresponding concentrations. Some samples require preparation prior to direct examination, as they may be "invisible" to the spectrophotometer initially. These colorimetric analysis techniques utilize a dying reagent, which acts to increase the absorbance of the sample. (Congdon, et.al 1993)
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