Genetic engineering of plant cells
Summary :
Table of Contents
- Introduction .
- Agrobacterium tumefaciens- mediated transformation.
- Genetic engineering.
- Direct gene transfer into plants.
- Viruses as gene vectors.
- Conclusion.
Abstract
In this text, we shall see how selection data have been used to assist the design of experiments on the modification of plants using recombinant DNA (genetic engineering). It is evident that in some situations the potential of in vitro selection is limited by a number of difficulties. It is partly because of these difficulties that genetic engineering methods are assuming an increasingly important role in the modification of plants. We now consider some of these methods.The plant pathogen Agrobacterium tumefaciens causes crown gall disease in higher plants. Infection by the bacterium commonly results in the formation of timorous outgrowths, the morphology of which depends on the plant species. The bacterium is now known to possess a large (95-160 Md) tumour-inducing (Ti) plasmid, part of which (the T-DNA) is transferred and incorporated into the host genome during infection.
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