Using UV-vis spectroscopy to determine the kinetics of bromophenol blue
Summary :
Table of Contents
- Introduction
- Results
- Discussion
- Conclusion
- References
Abstract
The purpose of this lab is to determine the rate constant and order of reaction for an acid-base indicator, bromophenol blue. bromophenol blue (BP) displays a color change from yellow to purple in the pH range of 3 to 4.5. In strong alkaline solutions, the dye becomes unstable and decomposes due to a reaction with hydroxide ions, as follows: . UV-vis spectroscopy will be used to record the absorbances at the wavelength of maximum absorbance.
The full differential rate law can be derived from the above reaction: , where k is the rate constant and x and y are the orders of reaction for bromophenol blue and the hydroxide ions respectively.1 However, a large excess of hydroxide will be used, allowing the concentration to remain constant throughout the experiment and allowing one to simplify the equation with respect to BP. The rate law for the disappearance of bromophenol blue is derived: .
The full differential rate law can be derived from the above reaction: , where k is the rate constant and x and y are the orders of reaction for bromophenol blue and the hydroxide ions respectively.1 However, a large excess of hydroxide will be used, allowing the concentration to remain constant throughout the experiment and allowing one to simplify the equation with respect to BP. The rate law for the disappearance of bromophenol blue is derived: .
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